RT Journal Article
SR Electronic
T1 Down-Regulation of the Novel Gene <em>Melastatin</em> Correlates with Potential for Melanoma Metastasis
JF Cancer Research
JO Cancer Res
FD American Association for Cancer Research
SP 1515
OP 1520
VO 58
IS 7
A1 Duncan, Lyn M.
A1 Deeds, Jim
A1 Hunter, John
A1 Shao, Jing
A1 Holmgren, Lisa M.
A1 Woolf, Elizabeth A.
A1 Tepper, Robert I.
A1 Shyjan, Andrew W.
YR 1998
UL http://cancerres.aacrjournals.org/content/58/7/1515.abstract
AB We have used differential cDNA display to search for genes whose expression correlates with an aggressive phenotype in variants of the B16 murine melanoma line, B16-F1 and B16-F10. This analysis identified a novel gene, termed melastatin, that is expressed at high levels in poorly metastatic variants of B16 melanoma and at much reduced levels in highly metastatic B16 variants. Melastatin was also found to be differentially expressed in tissue sections of human melanocytic neoplasms. Benign nevi express high levels of melastatin, whereas primary melanomas showed variable melastatin expression. Melastatin transcripts were not detected in melanoma metastases. Within the set of human primary cutaneous melanomas examined, melastatin expression appeared to correlate inversely with tumor thickness. The expression pattern observed suggests that loss of melastatin expression is an indicator of melanoma aggressiveness. ©1998 American Association for Cancer Research.