PT  - JOURNAL ARTICLE
AU  - Lu, Cai-De
AU  - Altieri, Dario C.
AU  - Tanigawa, Nobuhiko
TI  - Expression of A Novel Antiapoptosis Gene, Survivin, Correlated with Tumor Cell Apoptosis and p53 Accumulation in Gastric Carcinomas
DP  - 1998 May 01
TA  - Cancer Research
PG  - 1808--1812
VI  - 58
IP  - 9
4099  - http://cancerres.aacrjournals.org/content/58/9/1808.short
4100  - http://cancerres.aacrjournals.org/content/58/9/1808.full
SO  - Cancer Res1998 May 01; 58
AB  - A novel inhibitor of apoptosis designated survivin has recently been found in many common human cancers but not in normal tissues. A potential distribution of survivin in gastric cancer and its implication for apoptosis inhibition have been investigated. Recombinant survivin expressed in Escherichia coli as a glutathione S-transferase fusion protein was used to raise a novel panel of mouse monoclonal antibodies. In an immunohistochemical analysis of 174 cases of gastric carcinomas (stages I–III), anti-survivin monoclonal antibody 8E2 (IgG1) reacted with 34.5% of cases (60 of 174 cases) with a variable number of tumor cells stained (20–100%). In contrast, no expression of survivin in neighboring normal tissues was observed. When stratified for p53 and bcl-2 expression and apoptotic index, the expression of survivin significantly segregated with p53- and bcl-2-positive cases [56.1 versus 15.2% (P = 0.001) and 69.2 versus 31.6% (P = 0.006), respectively] and with a decreased apoptotic index as compared with that of survivin-negative tumors (0.97 ± 0.64 versus 0.62 ± 0.39%, P < 0.001). These data identify a role for survivin in promoting aberrantly increased cell viability in gastric cancer and suggest a potential correlation between accumulated p53 and survivin expression in neoplasia. ©1998 American Association for Cancer Research.