RT Journal Article
SR Electronic
T1 Cyclooxygenase-2 Expression Is Up-Regulated in Human Pancreatic Cancer
JF Cancer Research
JO Cancer Res
FD American Association for Cancer Research
SP 987
OP 990
VO 59
IS 5
A1 Tucker, Olga N.
A1 Dannenberg, Andrew J.
A1 Yang, Eun K.
A1 Zhang, Fan
A1 Teng, Lisong
A1 Daly, John M.
A1 Soslow, Robert A.
A1 Masferrer, Jaime L.
A1 Woerner, Bryan M.
A1 Koki, Alane T.
A1 Fahey, Thomas J.
YR 1999
UL http://cancerres.aacrjournals.org/content/59/5/987.abstract
AB A large body of evidence suggests that cyclooxygenase-2 (COX-2) is important in gastrointestinal cancer. The purpose of this study was to determine whether COX-2 was expressed in adenocarcinoma of the human pancreas. Quantitative reverse transcription-PCR, immunoblotting, and immunohistochemistry were used to assess the expression of COX-2 in pancreatic tissue. Levels of COX-2 mRNA were increased by >60-fold in pancreatic cancer compared to adjacent nontumorous tissue. COX-2 protein was present in 9 of 10 cases of adenocarcinoma of the pancreas but was undetectable in nontumorous pancreatic tissue. Immunohistochemical analysis showed that COX-2 was expressed in malignant epithelial cells. In cultured human pancreatic cancer cells, levels of COX-2 mRNA and protein were induced by treatment with tumor-promoting phorbol esters. Taken together, these results suggest that COX-2 may be a target for the prevention or treatment of pancreatic cancer. ©1999 American Association for Cancer Research.