PT  - JOURNAL ARTICLE
AU  - Guo, Qingbin M.
AU  - Malek, Renae L.
AU  - Kim, Sunkyu
AU  - Chiao, Chia
AU  - He, Mei
AU  - Ruffy, Mauro
AU  - Sanka, Krishna
AU  - Lee, Norman H.
AU  - Dang, Chi V.
AU  - Liu, Edison T.
TI  - Identification of c-Myc Responsive Genes Using Rat cDNA Microarray
DP  - 2000 Nov 01
TA  - Cancer Research
PG  - 5922--5928
VI  - 60
IP  - 21
4099  - http://cancerres.aacrjournals.org/content/60/21/5922.short
4100  - http://cancerres.aacrjournals.org/content/60/21/5922.full
SO  - Cancer Res2000 Nov 01; 60
AB  - c-Myc functions through direct activation or repression of transcription. Using cDNA microarray analysis, we have identified c-Myc-responsive genes by comparing gene expression profiles between c-myc null and c-myc wild-type rat fibroblast cells and between c-myc null and c-myc null cells reconstituted with c-myc. From a panel of 4400 cDNA elements, we found 198 genes responsive to c-myc when comparing wild-type or reconstituted cells with the null cells. The plurality of the named c-Myc-responsive genes that were up-regulated, including 30 ribosomal protein genes, are involved in macromolecular synthesis and metabolism, suggesting a major role of c-Myc in the regulation of protein synthetic and metabolic pathways. When ectopically overexpressed, c-Myc induced a different and smaller set of c-Myc-responsive genes as compared with the physiologically expressed c-Myc condition. Thus, these results from expression profiling suggest a new primary function for c-Myc and raise the possibility that the physiological and transforming functions of c-myc may be separable.