RT Journal Article SR Electronic T1 Trp-p8, a Novel Prostate-specific Gene, Is Up-Regulated in Prostate Cancer and Other Malignancies and Shares High Homology with Transient Receptor Potential Calcium Channel Proteins JF Cancer Research JO Cancer Res FD American Association for Cancer Research SP 3760 OP 3769 VO 61 IS 9 A1 Tsavaler, Larisa A1 Shapero, Michael H. A1 Morkowski, Stan A1 Laus, Reiner YR 2001 UL http://cancerres.aacrjournals.org/content/61/9/3760.abstract AB We have identified and cloned a novel gene, trp-p8, by screening a prostate-specific subtracted cDNA library. The 5694-bp cDNA has a 3312-bp open reading frame, which codes for a 1104 amino acid putative protein with seven transmembrane domains. The predicted protein revealed significant homology with the transient receptor potential (trp) family of Ca2+ channel proteins. Northern blot analysis indicated that trp-p8 expression within normal human tissues is mostly restricted to prostate epithelial cells. In situ hybridization analysis showed that trp-p8 mRNA expression was at moderate levels in normal prostate tissue and appears to be elevated in prostate cancer. Notably, trp-p8 mRNA was also expressed in a number of nonprostatic primary tumors of breast, colon, lung, and skin origin, whereas transcripts encoding trp-p8 were hardly detected or not detected in the corresponding normal human tissues. ©2001 American Association for Cancer Research.