RT Journal Article
SR Electronic
T1 High-Resolution Genome-Wide Mapping of Genetic Alterations in Human Glial Brain Tumors
JF Cancer Research
JO Cancer Res
FD American Association for Cancer Research
SP 4088
OP 4096
DO 10.1158/0008-5472.CAN-04-4229
VO 65
IS 10
A1 Bredel, Markus
A1 Bredel, Claudia
A1 Juric, Dejan
A1 Harsh, Griffith R.
A1 Vogel, Hannes
A1 Recht, Lawrence D.
A1 Sikic, Branimir I.
YR 2005
UL http://cancerres.aacrjournals.org/content/65/10/4088.abstract
AB High-resolution genome-wide mapping of exact boundaries of chromosomal alterations should facilitate the localization and identification of genes involved in gliomagenesis and may characterize genetic subgroups of glial brain tumors. We have done such mapping using cDNA microarray-based comparative genomic hybridization technology to profile copy number alterations across 42,000 mapped human cDNA clones, in a series of 54 gliomas of varying histogenesis and tumor grade. This gene-by-gene approach permitted the precise sizing of critical amplicons and deletions and the detection of multiple new genetic aberrations. It has also revealed recurrent patterns of occurrence of distinct chromosomal aberrations as well as their interrelationships and showed that gliomas can be clustered into distinct genetic subgroups. A subset of detected alterations was shown predominantly associated with either astrocytic or oligodendrocytic tumor phenotype. Finally, five novel minimally deleted regions were identified in a subset of tumors, containing putative candidate tumor suppressor genes (TOPORS, FANCG, RAD51, TP53BP1, and BIK) that could have a role in gliomagenesis. ©2005 American Association for Cancer Research.