RT Journal Article
SR Electronic
T1 Identification of <em>OTX2</em> as a Medulloblastoma Oncogene Whose Product can be Targeted by All-<em>Trans</em> Retinoic Acid
JF Cancer Research
JO Cancer Res
FD American Association for Cancer Research
SP 919
OP 924
VO 65
IS 3
A1 Di, Chunhui
A1 Liao, Shaoxi
A1 Adamson, David C.
A1 Parrett, Timothy J.
A1 Broderick, Daniel K.
A1 Shi, Qun
A1 Lengauer, Christoph
A1 Cummins, Jordan M.
A1 Velculescu, Victor E.
A1 Fults, Daniel W.
A1 McLendon, Roger E.
A1 Bigner, Darell D.
A1 Yan, Hai
YR 2005
UL http://cancerres.aacrjournals.org/content/65/3/919.abstract
AB Through digital karyotyping of permanent medulloblastoma cell lines, we found that the homeobox gene OTX2 was amplified more than 10-fold in three cell lines. Gene expression analyses showed that OTX2 transcripts were present at high levels in 14 of 15 (93%) medulloblastomas with anaplastic histopathologic features. Knockdown of OTX2 expression by siRNAs inhibited medulloblastoma cell growth in vitro, whereas pharmacologic doses of all-trans retinoic acid repressed OTX2 expression and induced apoptosis only in medulloblastoma cell lines that expressed OTX2. These observations suggest that OTX2 is essential for the pathogenesis of anaplastic medulloblastomas and that these tumors may be amenable to therapy with all-trans-retinoic acid. ©2005 American Association for Cancer Research.