PT  - JOURNAL ARTICLE
AU  - Luo, Yunping
AU  - Zhou, He
AU  - Mizutani, Masato
AU  - Mizutani, Noriko
AU  - Liu, Cheng
AU  - Xiang, Rong
AU  - Reisfeld, Ralph A.
TI  - A DNA Vaccine Targeting Fos-Related Antigen 1 Enhanced by IL-18 Induces Long-lived T-Cell Memory against Tumor Recurrence
AID  - 10.1158/0008-5472.CAN-04-3120
DP  - 2005 Apr 15
TA  - Cancer Research
PG  - 3419--3427
VI  - 65
IP  - 8
4099  - http://cancerres.aacrjournals.org/content/65/8/3419.short
4100  - http://cancerres.aacrjournals.org/content/65/8/3419.full
SO  - Cancer Res2005 Apr 15; 65
AB  - A novel vaccination strategy induced specific CD8+ T cell–mediated immunity that eradicated spontaneous and experimental pulmonary cancer metastases in syngeneic mice and was also effective in a therapeutic setting of established breast cancer metastases. This was achieved by targeting transcription factor Fos-related antigen 1(Fra-1), overexpressed by many tumor cells, with an ubiquitinated DNA vaccine against Fra-1, coexpressing secretory IL-18. Insight into the immunologic mechanisms involved was provided by adoptive transfer of T lymphocytes from successfully immunized BALB/c mice to syngeneic severe combined immunodeficient (SCID) mice. Specifically, long-lived T memory cells were maintained dormant in nonlymphoid tissues by IL-18 in the absence of tumor antigen. Importantly, a second tumor cell challenge of these SCID mice restored both, robust tumor-specific cytotoxicity and long-lived T-cell memory, capable of eradicating established pulmonary cancer metastases, suggesting that this vaccine could be effective against tumor recurrence. ©2005 American Association for Cancer Research.