Table 1

Summary of the 33 differentially methylated clones isolated by MCA-RDA

CloneSize (bp)%GCCpG/GpCCpG islandaBlast homologyChromosome mapGenbank accession no.
MINT1528560.6YesNone5q13-14AF135501
MINT2562500.8YesNone2p22-21AF135502
MINT3563551YesHuman EST1p34-35AF135503
MINT4481600.8YesNone15q25-26AF135504
MINT5852460.5YesbHuman CpG clonec14q21-22AF135505
MINT6400590.6YesNone12q14-15AF135506
MINT7481490.9YesHuman genomic DNAd6p21-22AF135507
MINT8617460.5YesbPAX6 enhancer11p.13AF135508
MINT9605540.3NoNone1p34-35AF135509
MINT10608490.6YesNone9q34–terAF135510
MINT11636480.5YesbVersican5q12-13AF135511
MINT12552490.6YesHuman CpG clone7q31-32AF135512
MINT13313600.9YesLINE1NDeAF135513
MINT14620540.4YesbNone10p13-15AF135514
MINT15641530.7YesNone11p12-13AF135515
MINT16664620.5Yesbα-Tubulin2qAF135516
MINT17490540.7YesNone6AF135517
MINT18435580.1NoNoneNDAF135518
MINT19443550.2NoNoneNDAF135519
MINT20510670.8YesMouse OTPNDAF135520
MINT21410620.4NoHuman genomic DNA22q13AF135521
MINT22438600.9YesNone10p12AF135522
MINT23414640.9YesCSX5q34-35AF135523
MINT24523670.7YesHuman EST3p25-26AF135524
MINT25339600.7YesHuman genomic DNA22q11AF135525
MINT26591580.8YesHuman CpG clone7q11AF135526
MINT27242740.7YesNoneNDAF135527
MINT28463581YesRibosomal RNA geneNDAF135528
MINT29429600.7YesHuman CpG clone7q11AF135529
MINT30536650.5YesNone20q11AF135530
MINT31673650.8YesHuman genomic DNA17q21AF135531
MINT32464661YesNone20q13AF135532
MINT33139650.8YesNoneNDAF135533
  • a The presence of CpG islands was determined based on criteria described previously (21) : minimum length, 200 bp; GC content, >50%; CpG/GpC, >0.5.

  • b Only one portion of the clone qualifies as a CpG island.

  • c Randomly sequenced clones from a CpG island library.

  • d Regions sequenced as part of the human genome project.

  • e ND, not determined.