Table 1

Effect of purine analogues on CAM vascularization

Methylcellulose discs were implanted onto the CAM of 8-day embryos (20 animals per group). Vascularization and fibroblast density were quantiated at day 12 by a planimetric method of “point counting.”
TreatmentMicrovessel densityFibroblast density
Intersection points (mean ± SD)Area (% of total)Intersection points (mean ± SD)Area (% of total)
Control (vehicle)8.0 ± 1.85.522 ± 415.2
AD (25 nmol/implant)7.0 ± 1.54.920 ± 213.9
2-AP (5 nmol/implant)7.0 ± 0.84.924 ± 316.7
6-MMP (5 nmol/implant)7.5 ± 1.25.221 ± 314.5
6-MMPR (5 nmol/implant)1.0 ± 0.3a0.719 ± 213.2
Anti-FGF2 Abb(400 ng/implant)3.0 ± 0.7a2.05 ± 1c3.6
  • a P < 0.001 versus control, AD, 2-AP, and 6-MMP.

  • b Ab, antibody.

  • c P < 0.001 versus all of the other groups.