Table 1

EC50 values of various eicosanoids to induce proliferation in COX-depleted HCA-7 cells

Proliferation was quantified by measuring [3H]thymidine incorporation in cells 21 h after treatment with PGs. The EC50 represents the molar concentration of a particular PG required to restore proliferation to 50% of that measured in antisense COX-2-transfected HCA-7 cells grown in the absence of Tc.
CompoundEC50 (m)
PGH24.0 × 10−7
U46619 (TxA2/PGH2 agonist)9.2 × 10−8
PGF1.0 × 10−6
PGE24.5 × 10−7
PGA23.8 × 10−8
PGB21.0 × 10−7
PGD23.7 × 10−7
PGJ27.2 × 10−10
Δ12-PGJ21.2 × 10−8
15-Deoxy-Δ12,14-PGJ29.2 × 10−10