Frameshift mutations in MMP+ endometrial tumors and cell lines
| hMLH1a methylation | hMSH3b (A)8 | hMSH6b (C)8 | BAXb (G)8 | IGFIIRb (G)8 | caspase-5b (A)10 | TGFβRIIb (A)10 | |
|---|---|---|---|---|---|---|---|
| MMP+ endometrial tumor cell linesc | |||||||
| AN3CA | + | − | +d | − | − | +d | − |
| RL95-2 | − | +d | +u | +d | − | +d | − |
| SK-UT-1 | − | − | − | +u | +u | +d | − |
| SK-UT-1B | − | − | − | +u | +u | +d | − |
| HEC-1A | − | − | ++d,d | − | ++u,uu | − | − |
| HEC-1B | − | − | ++d,d | − | ++u,uu | − | − |
| MMP+ endometrial tumorsd | |||||||
| ET9 | − | − | − | +d | − | − | − |
| ET24 | − | +d | − | +d | − | − | − |
| ET39 | − | − | − | +d | − | +d | − |
| ET45 | − | − | − | − | − | +d | − |
| ET46 | − | +d | − | +d | − | +d | − |
| ET67 | + | − | − | +d | − | − | − |
| ET68 | − | − | − | +d | − | − | − |
| ET69 | + | − | +d | +d | − | − | − |
| ET70 | + | − | − | − | − | − | − |
| ET83 | + | ++d,d | − | − | − | +d | − |
| ET86 | − | − | +d | − | − | − | − |
| ET100 | − | ++d,d | − | − | − | − | − |
| ET106 | − | − | − | +d | − | − | − |
| ET132 | − | − | − | +d | − | − | − |
| ET153 | − | − | +u | − | − | − | − |
| ET156 | − | − | − | − | − | +d | − |
| ET162 | + | ++d,d | − | − | − | − | +d |
| ET167 | + | − | − | +d | − | − | − |
a −, not methylated; +, methylated.
b −, wild type; +, mutant; +u, 1-bp insertion; ++u,uu, homozygous insertions of 1 and 2 bp; +d, 1-bp deletion; ++d,d, homozygous deletion of 1 bp.
c Hypermethylation was absent in three MMP− endometrial (Hs34.T, Hs248.T, and Hs825.T) and colon SW620 cell lines and in MMP+ colon carcinoma (LoVo, LS411N, DLD-1, LS174, LS180, and HCT116) and prostate carcinoma DU-145 cell lines. The three MMP− endometrial cell lines were negative for mutations in all the target genes. Additional MMP− cell lines were also negative for mutations, including: 16 from colon (SW620, SW403, SK-CO-1, NCI-H747, NCI-H508, Caco-2, HT-29, BM314, CHC-Y1, COLO201, RCM-1, SW837, SW1417, WiDr, and COCO320DM); 9 from pancreas (MiAPaCa-2, Capan-1, Capan-2, PANC-1, AsPC-1, HPAF, Hs766T, BxPC3, and Hs578T); 7 from stomach (AZ521, JRST, KATOIII, MKN 1, MKN28, MKN 45, and MKN74); 2 from lung (A549 and SK-LU-1); and 1 each from small intestine (HuTu80), ovary (PA-1), and breast (MDA-MB-468).