Table 2

Effects of cyclin D1 suppression on relative TS and TP gene expression

Cell linesRelative gene expression
TSaTPa
COLO-35752 ± 0.30.17 ± 0.04
Sham-C38 ± 4.00.27 ± 0.01
CC719 ± 4.2b0.45 ± 0.02b
CC925 ± 4.1b0.43 ± 0.07b
PANC-135 ± 0.10.57 ± 0.01
Sham46 ± 2.60.62 ± 0.03
C531 ± 2.21.45 ± 0.05b
C3427 ± 0.6b2.38 ± 0.06b
  • a Data are expressed as the ratio of the threshold cycle of the internal standard β-actin and the threshold cycle of the gene of interest and are the means ± SD from at least three separate determinations.

  • b Significant difference (P < 0.05) by comparison with the corresponding parental and sham-transfected cells.