Table 1

Protocol 1: Cytotoxicity of sequential treatment with MTA followed by GEM in vitro

Cells were incubated with drug-vehicle or MTA 24 h before the addition of GEM for 24 h. Cells were cloned as described in “Materials and Methods.” IC50, IC90, and IC99 values were calculated from median effect dose-plots of the data in Fig. 4 <$REFLINK> (3) . The numbers in parentheses indicate the fold decrease in the corresponding value from GEM alone.
GEMMTA (0.1 μm) → GEMMTA (0.3 μm) → GEM
IC50m)0.071 ± 0.0110.074 ± 0.0090.032 ± 0.009 (2.2)
IC90m)0.648 ± 0.2290.205 ± 0.018 (3.2)0.094 ± 0.019 (6.9)
IC99m)11.65 ± 5.930.638 ± 0.071 (18.3)0.331 ± 0.070 (35.2)