Protocol 3: Cytotoxicity of the sequential treatment with MTA followed by 1 h of GEM in vitro
| Subconfluent monolayers were treated with MTA at 0, 0.3, or 1 μm for 24 h, followed by GEM for 1 h. Data are expressed as percentage of colonies formed relative to untreated samples. Values are averaged from three independent experiments ± SE. | ||||||
|---|---|---|---|---|---|---|
| Colony formation, % untreated | ||||||
| GEM, μm | No MTA | MTA (0.3 μm) | MTA (1 μm) | |||
| 0 | 100 | 79 ± 5 | 68 ± 8 | |||
| 0.3 | 71 ± 7 | 61 ± 8 | 50 ± 8 | |||
| 1 | 71 ± 14 | 40 ± 4 | 45 ± 12 | |||
| 3 | 61 ± 6 | 37 ± 5 | 30 ± 6 | |||
| 10 | 51 ± 2 | 31 ± 4 | 21 ± 4 | |||
| 30 | 63 ± 3 | 38 ± 3 | 30 ± 8 | |||