Table 2

BM-DCs injected directly into tumors induce IFN-γ production by lymphoid cells harvested from the draining lymph node and spleen

GroupLymph nodesaSpleena
IL-4bIFN-γbIL-4IFN-γ
HBSS<32115 ± 6<3221 ± 3
BM-DC-Tr.(−)90 ± 10c1,373 ± 112d<32476 ± 98d
BM-DC-Zeo155 ± 21c1,316 ± 81d<32584 ± 36d
BM-DC-IL-1280 ± 10c16,130 ± 83e<327,593 ± 1,080e
Fibroblast-IL-12<32189 ± 12c<3243 ± 4c
  • a Draining lymph nodes and spleen were harvested 7 days after intratumoral (MCA205) injection with HBSS, nontransduced, or Zeo- or IL-12-transduced BM-DCs or IL-12-transduced syngeneic fibroblasts. Cells were cocultured with irradiated MCA205 (5000 rads) in a 24-well plate for 36 h. The supernatants were assessed with ELISA.

  • b Data represent the mean ± SD pg/106 cells/36 h of duplicated samples.

  • c Significant (P < 0.05) versus HBSS.

  • d Significant (P < 0.05) versus HBSS and IL-12-transduced fibroblasts.

  • e Significant (P < 0.05) versus all other groups.