Table 1

Analysis of ACF by densitometry after PCR amplification of the androgen receptor

Patient no.ACF (trial no.)SizeaNo. of crypts dissectedbRAAcRAI(N)dRAI(E)eLocation of ACFPathology of ACF
1897A (1)11060.810.850Ascending colonMild dysplasia
A (2)60.890.740.18
2101A (1)2770.920.800.09Ascending colonMild dysplasia
A (2)70.880.750.30
221A (1)7281.010.700Ascending colonMild dysplasia
A (2)81.050.710
B (1)22580Ascending colonAtypia
B (2)80
179A (1)6470.730.860.04Ascending colonAtypia
A (2)71.110.790
1807D (1)1950.900.960.11Transverse colonMild dysplasia
D (2)51.390.600
1429A (1)9180.650.910.06SigmoidAtypia
A (2)80.700.800.13
B (1)20080.24SigmoidAtypia
B (2)80.19
638B (1)5051.091.000.27RectumMild dysplasia
B (2)50.730.870
1900A (1)2570.760.870.11RectumMild dysplasia
A (2)70.730.700
B (1)4170.13RectumMild dysplasia
B (2)70
Total
8116.8f0.900.810.08
±1.1±0.20±0.11±0.10
  • a Number of crypts per ACF.

  • b Number of crypts microdissected within an ACF.

  • c Allele amplification ratio for normal colonic tissue (mixture of stromal and epithelial cells).

  • d Allele inactivation ratio after digestion with HpaII for normal colonic tissue (mixture of stromal and epithelial cells).

  • e Allele inactivation ratio after digestion with HpaII for epithelial cells in five to eight crypts from an ACF.

  • f Mean ± SD.