Table 2

Analysis of normal crypts by densitometry after PCR amplification of the androgen receptor

Patient no.Trial no.No. of crypts dissectedaRAAbRAI(N)cRAI(E)d
1897170.600.910
280.910.880
2101180.900.830.79
280.820.750.77
221181.000.810.80
281.000.790.64
179180.780.760.81
281.060.880.98
1807151.120.820.45
250.880.980.80
1429180.960.630.86
280.630.790.95
638170.850.960.95
270.860.910.70
1900180.600.600.72
280.700.690.78
Total
87.4e0.850.810.79f
±1.0±0.16±0.11±0.14
  • a Number of normal crypts microdissected.

  • b Allele amplification ratio for normal colonic tissue (mixture of stromal and epithelial cells).

  • c Allele inactivation ratio after digestion with HpaII for normal colonic tissue (mixture of stromal and epithelial cells).

  • d Allele inactivation ratio after digestion with HpaII for epithelial cells in five to eight normal crypts.

  • e Mean ± SD.

  • f Mean ± SD of samples that appear polyclonal, i.e., excluding those from patient 1897.