Table 1

Detection of nuclear aggregates in ALT cell lines

Cell nameCell type (immortalizing agent)Telomere maintenance mechanismNuclear aggregates
GM847Fibroblast (SV40)ALT+
WI38-VA13/2RAFibroblast (SV40)ALT+a
IIICF-T/B1Fibroblast (SV40)ALT+
JFCF-6/T.1RFibroblast (SV40)ALT+
SUSM-1Fibroblast (chemical carcinogen)ALT+
KMST-6Fibroblast (γ irradiation)ALT+
IIICF/a2Fibroblast (spontaneous)ALT+
IIICF/b4Fibroblast (spontaneous)ALT+
IIICF/b5Fibroblast (spontaneous)ALT+
IIICF/cFibroblast (spontaneous)ALT+
BET-3MEpithelial (SV40)ALT+
SAOS-2OsteosarcomaALT+
BFT-3BFibroblast (SV40)Telomerase
JFCF-6/T.1CFibroblast (SV40)Telomerase
BET-3bEpithelial (SV40)Telomerase
293Epithelial (adenovirus)Telomerase
HeLaCervical carcinomaTelomerase
MCF-7Breast adenocarcinomaTelomerase
Bre-80Breast epithelial strain
MRC-5Fibroblast strain
HFF5Fibroblast strain
JFCF-6/T.1RFibroblast, preimmortal SV40-transformed
IIICF/cFibroblast, preimmortal
  • a This cell line contained smaller nuclear aggregates than the other 11 ALT lines.