Table 2

Molecular characterization of RR expression in the KB-Gem clonea

M1M2
CellsRelative amounts of M1b mRNARelative amounts of M1c proteinRelative amounts of M2b mRNARelative amounts of M2c protein
KB wild-type1.01.01.01.0
KB-Gem1.21.09.02.0
HURS1.51.210.03.0
  • a Quantitative values are the means of three experiments.

  • b Determined from phosphoimaging measurements of the band hybridizing with M1 or M2 probes with mRNA from KB-Gem clone and HURS clones, relative to measurement of the band hybridizing with RNA from wild-type cells. Results were corrected according to a fixed control value of β-actin mRNA.

  • c Determined the densitometric measurements of the crude extracted protein blot with M1 or M2 antibodies from KB-Gem clone and HURS clones, relative to protein from wild-type cells.