Table 3

Relative changes in RR activity and dNTP pools in the KB-Gem clone

dNTP poolsb
Relative RR enzyme activityadATPdCTP
KB wild-type111
KB-Gem2.3×
HURS6.7×15×
  • a Enzyme activity was determined as described in “Materials and Methods” and was expressed as activity relative to the KB wild-type. Enzyme activity is expressed as nmol CDP reduced/h/mg protein.

  • b Relative values represent the averages of data from three assays, each performed in duplicate.