Table 2

Comparison of TS levels in WiDr mono- and multilayered cells measured by different methods

Values are the means ± SE of at least three separate experiments. The various TS assays were performed as described in “Materials and Methods.”
TS assayWiDr cellsWiDr/F cells
MonolayerMultilayerMonolayerMultilayer
TS catalytic activity (pmol/h/106 cells)
 1 μm dUMP33 ± 236 ± 1227 ± 211 ± 2.9
 10 μm dUMP131 ± 258 ± 24148 ± 1224 ± 6.0
 ratio, 10:14.01.65.52.2
TS in situ (fmol/h/106 cells)171 ± 16117 ± 258 ± 763 ± 7
FdUMP binding (fmol/106 cells)65 ± 2205 ± 6122 ± 482 ± 15
Western blot (ng/106 cells)0.50.30.30.2