Table 1

Enhanced immune response and antitumor response of rV-CEA/TRICOM in CEA transgenic mice

C57BL/6 CEA-transgenic mice (five per group) were vaccinated via skin scarification with buffer or vaccinia recombinant (107 pfu) one time on day 0. On day 30, two mice were killed, and splenic T cells were analyzed for T-cell proliferative responses. Each value represents the stimulation index of the mean cpm of triplicate samples versus media. SD never exceeded 10%. On day −4, three mice per group were given 4 × 105 MC38 colon carcinoma cells expressing CEA. Tumor volume is given at days 14 and 35 after vaccination.
Stimulation indexTumor volume
Con AOvalCEACEA
Immunogen(5 μg/ml)(100 μg/ml)(100 μg/ml)(25 μg/ml)Day 14Day 35
HBSS1091.01.32.0698 ± 9283674 ± 3107
rV-CEA1230.94.94.0259 ± 01112 ± 1685
rV-CEA/B7-1931.37.14.3150 ± 2362696 ± 1936
rV-CEA/TRICOM1111.119.215.90 ± 00 ± 0