Table 2

Effect of T-cell depletion: Recovery of T cells and B-CLL from human→mouse chimera

Patient no.UntreatedTreated with OKT3CLL ratiob
Number of miceCLL cellsT cellsNumber of miceCLL cellsT cells
%aTotal (× 104)%aTotal (× 104)%aTotal (× 104)%aTotal (× 104)
Early stage
  141.0 ± 0.3c0.7 ± 0.3c20.5 ± 9.328.8 ± 23.5c248.0 ± 13.085.0 ± 67.0121.0
  233.0 ± 0.6c1.4 ± 0.2c37.0 ± 7.517.0 ± 1.7c220.0 ± 1.07.8 ± 3.60.7 ± 0.30.3 ± 0.35.6
  3637.6 ± 9.7c94.7 ± 44.320.8 ± 3.140.3 ± 15.8c675.6 ± 3.1641.5 ± 179.00.3 ± 0.21.1 ± 0.86.7
  411.02.041.088.0112.07.01.03.5
  5117.07.015.06.0273.5 ± 6.5118.0 ± 58.016.8
  6117.010.035.021.0138.030.03.0
  71041.3 ± 9.0c84.8 ± 30.4c8.5 ± 1.6c7.8 ± 1.9c867.6 ± 3.7162.1 ± 49.40.5 ± 0.20.6 ± 0.31.6
  8417.3 ± 9.410.6 ± 7.5c23.0 ± 7.1c18.1 ± 12.1250.5 ± 19.5177.5 ± 25.516.7
  9241.0 ± 4.039.0 ± 12.516.5 ± 4.5c18.0 ± 11.0245.0 ± 13.041.5 ± 36.51.1
 10245.0 ± 3.0c38.3 ± 19.04.0 ± 0.0c3.3 ± 1.5144.0189.24.9
 1132.0 ± 0.6c6.7 ± 2.760.7 ± 3.5c193.3 ± 58.1316.3 ± 3.56.0 ± 1.20.9
 Mean ± SE (P value)3720.3 ± 5.4 (0.005)26.8 ± 10.2 (0.03)25.6 ± 5.0 (0.0001)40.1 ± 16.8 (0.01)3044.6 ± 6.7133.3 ± 55.10.3 ± 0.10.2 ± 0.116.5 ± 10.5
Advanced stage
 12220.0 ± 5.01.5 ± 0.59.5 ± 1.5c0.6 ± 0.2c225.5 ± 9.57.5 ± 5.55.0
 13634.7 ± 5.3c48.8 ± 20.6c21.8 ± 2.6c29.2 ± 14.2c456.5 ± 2.3217.3 ± 67.40.5 ± 0.31.0 ± 0.74.4
 14d318.3 ± 8.6c7.1 ± 3.0c2.0 ± 0.6c0.9 ± 0.5355.3 ± 4.461.3 ± 24308.6
 15379.7 ± 3.5292.7 ± 108.44.2 ± 0.2c15.0 ± 5.0c584.8 ± 3.9265.8 ± 84.60.2 ± 0.20.6 ± 0.60.9
 16584.8 ± 2.8439.6 ± 257.80.4 ± 0.1c1.8 ± 1.0486.3 ± 6.7687.8 ± 138.50.2 ± 0.10.9 ± 0.30.9
 17229.5 ± 18.520.7 ± 18.07.0 ± 4.07.5 ± 1.5238.5 ± 5.0123.5 ± 57.52.5 ± 0.57.0 ± 1.06.2
 18d627.6 ± 4.3c27.7 ± 6.2c1.3 ± 0.3c1.0 ± 0.1c659.5 ± 5.8117.2 ± 14.90.1 ± 0.10.1 ± 0.14.2
 19122.015.011.07.0   
 20134.048.02.03.0   
 21240.0 ± 0.0c32.7 ± 14.32.5 ± 1.52.2 ± 1.830.3 ± 0.30.2 ± 0.20.0
 22264.5 ± 2.5c209.0 ± 157.80.3 ± 0.20.9 ± 0.820.0
 Mean ± SE (P value)3341.5 ± 7.3 (NSe)103.8 ± 43.8 (NS)5.6 ± 2.0 (0.01)6.3 ± 2.6 (0.04)3145.2 ± 10.6164.5 ± 72.60.4 ± 0.31.1 ± 0.83.4 ± 1.0
  • a Percentage of total cells in peritoneal wash.

  • b Total CLL count in OKT3-treated mice/total CLL count in untreated.

  • c P < 0.05 in comparison with the equivalent value in the treated group.

  • d T depletion was performed ex vivo by SRBC rosettes.

  • e NS, not significant.