Table 2

List of HLA-A*0301 binding peptides from HER-2/neu

A*0301 bindingb IC50 (nm)No. of cultures containing CTL reactive with:c
PeptideaSequencePeptideTumor cells
HER2[9681]KIRKYTMRR14.542d
HER2[9852]LVKSPNHVK22.910
HER2[9754]VLRENTSPK27.511
HER2[10753]KVLRENTSPK28.900
HER2[9673]ILIKRRQQK28.900
HER2[9167]ILWKDIFHK39.300
HER2[10141]QLRSLTEILK55.000
  • a Numbers in brackets represent peptide size and the position on the protein sequence (subscript).

  • b Measured as concentration (nm) of test peptide required for 50% inhibition of binding of the standard peptide. See “Materials and Methods” for details.

  • c Cultures were considered containing CTL when specific cytotoxicity towards antigen-containing target (peptide-pulsed or tumor cell line) was >20% above cytotoxicity with negative control target. Values represent the number of positive wells of 40 wells that were tested.

  • d Antigen specificity was not confirmed by cold target inhibition assay.