Table 1

PTEN gene transfer to ovarian cancer cells and quantified transcription levels of PTEN, PIK3CA, and adenovirus receptors

Cell lineGrowth inhibitiona,b (%)Transduction efficiencyb,c (%)PTENdPIK3CAdIntegrin subunitd
αvbβ3β5
MDAH 2774871001.85.12.423.41.0
TYK-nu82930.21.51.03.50.1
SW 626751000.11.20.70.40.4
NIH:OVCAR-375831.01.91.01.01.4
OV-106367700.51.20.50.80.3
SK-OV-362710.30.60.71.90.0
Caov-332440.11.00.30.20.1
ES-223121.22.30.42.50.4
MCAS1590.20.30.30.00.0
  • a The growth-inhibitory effects of AdCAPTEN infection were calculated as (x − y)/x (x, mean cell number 5 days after AdCA infection at 100 MOI; y, mean cell number 5 days after AdCAPTEN infection at 100 MOI).

  • b The correlations between transcription levels of Integrin αv and adenoviral transduction efficiencies or growth-inhibitory effects were both statistically significant (Integrin αv versus growth inhibition, P = 0.009, ρ = 0.924; Integrin αv versus transduction efficiency, P = 0.014, ρ = 0.869; Spearman’s rank correlation).

  • c Mean percentages of 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside-positive cells were measured microscopically 24 h after AdCALacZ infection at 100 MOI.

  • d The transcription levels of PTEN, PIK3CA, Integrin αv, Integrin β3, and Integrin β5 were examined using semiquantitative RT-PCR. The intensity of each band was calculated as a ratio against the intensity of the corresponding β2-microglobulin band by densitometric analysis.