Table 1

Phenotype of AUXB1 cells transfected with cDNAs encoding mouse FPGS speciesa

CellsDNA transfectedColonies formed per plate
α+αα+ minus glycine
Controls
 AUXB1None181 ± 180 ± 00 ± 0
 CHONone192 ± 19193 ± 5178
 FC2/2DHuman genomicb142 ± 13134 ± 9134 ± 22
 10-1pcDNA3162 ± 160 ± 00 ± 0
 10-2pcDNA3226 ± 100 ± 00 ± 0
cDNA transfectants
 Clone 1-4L1210 upstream177 ± 11172 ± 11186 ± 5
 Clone 1-6L1210 upstream172 ± 1365 ± 631 ± 1
 Clone 2-2L1210 downstream196 ± 7208 ± 110 ± 0
 Clone 2-7L1210 downstream251 ± 11254 ± 130 ± 0
 Clone 3-3Liver upstream193 ± 14188 ± 5173 ± 5
 Clone 3-5Liver upstream131 ± 10140 ± 9139 ± 8
 Clone 4-2Liver downstream102 ± 4111 ± 30 ± 0
 Clone 4-12Liver downstream194 ± 19189 ± 90 ± 0
  • a The indicated cDNAs were transfected into 5000 AUXB1 cells/100-mm dish and selected in α+ medium supplemented with 1.2 mg/ml G418, and multiple well-isolated colonies were picked and mass-cultured. Cloned transfectants that expressed FPGS were plated at 250 cells/100-mm dish in α+ medium, and the indicated media were applied after 16 h. Colonies were fixed and stained 10 days later.

  • b See Ref. 12 .