Table 2

CENU-lex-induced mutations at the p53 cDNA after in vitro treatment of plasmid pLS76 and passage into yIG397 cells

Mutant no.aMutation typebNucleotide position5′→3′ sequencec (NT strand)Amino acid changeCCNUd
Single bp substitutions
 9GC→AT263eAGcCCC→AGTCCA 88 Vf
 8, 116, 117GC→AT273TGgCC→TGaCCW 91 Stop
 77GC→TA293eCCcTT→CCaTTP 98 Hf
 34, 108GC→TA296eTTcCC→TTaCCS 99 Yf
 105GC→AT310ACcAG→ACtAGQ 104 Stop
 78AT→TA400TGtTT→TGaTTF 134 I
 39, 99GC→AT438TGgGT→TGaGTW 146 Stop
 94GC→AT451CAcCC→CAtCCP 151 S
 46GC→TA452ACcCC→ACaCCP 151 H
 12GC→TA454CCcCG→CCaCGP 152 T
 60GC→AT455CCcGC→CCtGCP 152 L
 30AT→TA484CCaTC→CCtTCI 162 F
 107GC→AT529GCcCC→GCtCCP 177 S
 96GC→AT535ACcAT→ACtATH 179 Y
 57, 111GC→AT538ATgAG→ATaAGE 180 K
 15GC→AT542GCgCT→GCaCTR 181 H
 125AT→TA613GtATT→GaATTY 205 N
 37AT→TA647TGtGG→TGaGGV 216 E
 95GC→AT730TGgGC→TGaGCG 244 S+
 115GC→AT733GCgGC→GCaGCG 245 S+
 14GC→AT734CGgCA→CGaCAG 245 D+
 56AT→TA736GCaTG→GCtTGM 246 L
 50GC→AT749GCcCA→GCtCAP 250 L
 32GC→AT754TCcTC→TCtTCL 252 F
 98AT→GC770ACtGG→ACcGGL 257 P
 97GC→AT796TGgGA→TGaGAG 266 R+
 27GC→AT797GGgAC→GGaACG 266 E
 109GC→TA820GTgTT→GTtTTV 274 F
 70GC→AT827TGcCT→TGtCTA 276 V+
 36GC→AT836TGgGA→TGaGAG 279 E+
 92GC→TA836TGgGA→TGtGAG 279 Vf
 45GC→CG843GAcCG→GAgCGD 281 E
 101GC→AT845CCgGC→CCaGCR 282 Q
 41GC→AT856AGgAA→AGaAAE 286 K
 124GC→AT916AGcGA→AGtGAR 306 Stop
 25AT→TA981TAtTT→TAaTTY 327 Stop
Other mutations
 42+1 A/T355–356A GCC A→A GtC CA119 frameshift
 64−1 G/C690ACc ACCACACC 231 frameshift
 47−1 A/T838aGA GACGAG AC280 frameshift
 1+1 A/T894–895GAG CTGGAG aCT299 frameshift
 100GC→AT263eAGcCC→AGtCCA 88 Vf
GC→TA796TGgGA→TGtGAG 266 Stop
 22GC→AT381TCcCC→TCtCCS 127 S
GC→AT749GCcCA→GCtCAP 250 L
  • a Independent mutants showing the same mutations are identified by their number.

  • b Mutations at the GC (or AT) bp are reported as induced by G- (or A-) targeted lesions.

  • c Lowercase letters, mutated bases; underlined letters, p53 codon.

  • d +, the same amino acid substitution was found among CCNU-induced p53 mutations (21) .

  • e Base in the p53 cDNA for which bp substitutions have not been found so far in human tumors (IARC p53 database: 9378 mutations; Ref. 29 ).

  • f Amino acid change not found so far in human tumors or cell lines (IARC p53 data base: 9378 mutations; Ref. 29 ).