Table 1

Analysis of mRNA expression, ET-1 production, and receptor expression

PCR productsRIAbBinding constants
Primary cellsStageTumor typeET-1ETAETBET-1 (pmol/106 cells)Kd (nm)Bmax
PMOV1IIISerous+a++0.56 ± 0.02NDND
PMOV2IIISerous++0.78 ± 0.04NDND
PMOV3IIIEndometrioid++0.80 ± 0.06NDND
PMOV4IIIMucinous+++0.51 ± 0.02NDND
PMOV5IIISerous++0.75 ± 0.04NDND
PMOV6IVSerous++0.72 ± 0.02NDND
OVP1IIIAdenocarcinoma+++0.59 ± 0.011.0 ± 0.118,504 ± 800
OVP2IIIMucinous++0.83 ± 0.010.8 ± 0.0522,000 ± 400
OVM1IVSerous+++0.48 ± 0.041.2 ± 0.120,800 ± 700
OVM2IIISerous++0.47 ± 0.010.9 ± 0.221,005 ± 950
  • a +, presence of product; −, absence of product; ND, result not determined.

  • b ET-1 concentrations in conditioned media collected after a 48-h culture are given as the meanNULLNULL ±NULL SD of three determinations.