Table 2

Polymorphism detected by PCR-SSCP analysis in Tsc1 locus

A. ExonPositionPolymorphism LEC/LEK WKAH BN F344
326 bp before the start of the codon initiatorC→G
914 bp after the donor site of intron 920 nt deletion
5′-CTTTGGTCTCCTGTGTTTGCAACTTCAT-3′
Codon 287CAC(His)→CAG(Gln)
1410 bp before the acceptor site of intron 13G→C
154 bp before the acceptor site of intron 14C→T
16Codon 674GTT(Val)→CTT(Leu)
23Codon 1027CGG(Arg)→TGG(Trp)
Codon 1106ATG(Met)→GTG(Val)
B. ExonPositionPolymorphism LEK/WKAH BN
20Codon 852CTC(Leu)→CTT(Leu)