Table 3

Mutations of Tsc1 and Tsc2 gene in rat RCs

No.StrainSexAge (wk)Tumor size (mm)HistologyTreatmentMutationsa
1F344M4710RC0.1% EHEN (1 wk × 3) + 0.01% KBrO3
2F344M4725RC0.1% EHEN (1 wk × 3) + 0.01% KBrO3Tsc2: exon 21 TGT→TGA Cys800→Stop
3F344M4720RC0.1% EHEN (1 wk × 3) + 0.01% KBrO3Tsc2: exon 10 TAT→TAA Tyr336→Stop
Tsc2: intron 11 donor site GT→GC
4(LEC × WKAH)F2F777RCDEN (100 mg/kg × 2)Tsc2: exon 19 CTC→CGC Leu713→Arg
5(LEC × F344)F2F7650RCDEN (100 mg/kg × 2)Tsc2: exon 33 TTG→TAG Leu1384→Stop
6(LEC × F344)F2M7710RCDEN (100 mg/kg × 2)
7(LEC × F344)F2M7625RCDEN (100 mg/kg × 2)Tsc2: exon 20 TTG→TAG Leu780→Stop
Tsc2: intron 27 acceptor site AG→AT
8(LEC × F344)F2M768RCDEN (100 mg/kg × 2)
9(F344 × LEC)F1M366RC0.05% EHEN (2 wk + 1 wk × 2)Tsc1: intron 16 donor site GT→GA
Tsc1: intron 16 donor site GT→GC
10(F344 × LEC)F1M411.5RC0.05% EHEN (2 wk + 1 wk × 2)Tsc2: exon 1 TTG→TAG Leu45→Stop
Tsc2: exon 9 AAC→AGC Asn314→Ser
Tsc2: exon 34 CTC→CGC Leu1508→Stop
11(F344 × LEC)F1M4112RC0.05% EHEN (2 wk + 1 wk × 2)
12(F344 × LEC)F1M428RC0.05% EHEN (2 wk + 1 wk × 2)Tsc2: exon 26 TTG→TAG Leu1020→Stop
13(F344 × LEC)F1M433RC0.05% EHEN (2 wk + 1 wk × 2)
14(F344 × LEC)F1M444RC0.05% EHEN (2 wk + 1 wk × 2)
15(F344 × LEC)F1M432RC0.05% EHEN (2 wk + 1 wk × 2)Tsc2: LOH
Tsc2: intron 34 donor site GT→GC
  • a Tsc2 mutations of samples 1–8 were reported previously (11) . Vh1 mutations were not detected by our PCR-SSCP analysis.