Table 1

The effect of 5α-DHT (10−10m) and human EGF (0.2 ng/ml) on protein kinase CK2 activity in LNCaP and PC-3 cells

Androgen and EGF treatment lasted 72 h; the control cells were maintained in the absence of these factors as described in “Materials and Methods.”
CellsCell fractionCK2 activity (nmol 32P/mg protein/h; mean ± SD)
Control5α-DHTh-EGFa5α-DHT + h-EGF
LNCaPCytosol9.7 ± 0.95.2 ± 0.46.9 ± 0.57.2 ± 0.6
NM6.0 ± 0.59.8 ± 0.89.2 ± 0.89.4 ± 0.9
PC-3Cytosol11.5 ± 0.812.3 ± 1.08.1 ± 0.77.8 ± 0.6
NM7.3 ± 0.57.0 ± 0.710.2 ± 0.910.9 ± 0.8
  • a h-EGF, human EGF.