The effect of 5α-DHT (10−10m) and human EGF (0.2 ng/ml) on protein kinase CK2 activity in LNCaP and PC-3 cells
Androgen and EGF treatment lasted 72 h; the control cells were maintained in the absence of these factors as described in “Materials and Methods.” | |||||
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Cells | Cell fraction | CK2 activity (nmol 32P/mg protein/h; mean ± SD) | |||
Control | 5α-DHT | h-EGFa | 5α-DHT + h-EGF | ||
LNCaP | Cytosol | 9.7 ± 0.9 | 5.2 ± 0.4 | 6.9 ± 0.5 | 7.2 ± 0.6 |
NM | 6.0 ± 0.5 | 9.8 ± 0.8 | 9.2 ± 0.8 | 9.4 ± 0.9 | |
PC-3 | Cytosol | 11.5 ± 0.8 | 12.3 ± 1.0 | 8.1 ± 0.7 | 7.8 ± 0.6 |
NM | 7.3 ± 0.5 | 7.0 ± 0.7 | 10.2 ± 0.9 | 10.9 ± 0.8 |
a h-EGF, human EGF.