Table 2

Demethylation in different regions of the hMLH1 promoter after 5-aza-2′-deoxycytidine treatment

Cell lineDemethylation extenta in different regions
AbBCD
C1a4%23%40%40%
VACO522%17%19%24%
RKO0%1%29%29%
C29%32%33%29%
  • a The extent of demethylation was calculated by averaging the percentage of meth-ylation of all CpG sites in the region before 5-aza-2′-deoxycytidine treatment subtracted by the average percentage of methylation after treatment. The concentrations of 5-aza-2′-deoxycytidine used to treat cell lines C1a, VACO5, RKO, and C were 5, 15, 10, and 5 μm, respectively.

  • b Regions A, B, C, and D are the same as in Table 1 <$REFLINK> .