Table 1

MORF4 sequence alleles

MORF4 sequence at polymorphic loci in tumor-derived cell lines, normal fibroblast counterparts (i.e., 3F fibroblasts were derived from the same patient as the BICR 3 line, and so forth) and “normal” material used for microcell transfer (F4)/transfection (cloned NheI fragment; see Ref. 7 ). Loci base numbering is as used in Fig. 2 <$REFLINK> . Where two bases are indicated at one position, the sequence was found to be heterozygous at this locus; i.e., two different allelic sequences are present in the same cells. The final column indicates lines that are heterozygous and not homo- nor hemizygous for MORF4 and the 4q33-q34 LOH locus in the vicinity of D4S408.
Base at polymorphic lociHeterozygous
49108115428615730865 MORF4 D4S408
Tumor lines
 BICR-3A/GTA/GGC/TA/TA/GYesYes
 BICR-6ATAGCAANoNo
 BICR-10AT/CAGCAAYesnNDa
 BICR-16GTAGTTGNoND
 BICR-18GTAGTTGNoNo
 BICR-19A/GT/CA/GGCA/TA/GYesNo
 BICR-22A/GTAGC/TA/TA/GYesNo
 BICR-31ACAGCAANoNo
 BICR-56A/GTAGC/TA/TA/GYesND
 BICR-63GTA/GG/ATTGYesYes
 BICR-68GTAG/ATTGYesYes
 BICR-78ACAGCAANoNo
 BICR-82ATAGCAANoNo
 SCC-4AT/CAGCAAYesND
 SCC-9GTA/GGTTGYesND
 SCC-12A/GT/CA/GGC/TA/TA/GYesND
 SCC-13A/GT/CAGCAAYesND
 SCC-15A/GTA/GGCA/TA/GYesND
 SCC-25ATAGCAANoND
 SV-HFKATAGCAANoND
 DOKACAGCAANoND
Fibroblasts
 3FA/GTA/GGC/TA/TA/GYesN/A
 6FAT/CAGCAAYesN/A
 18FGTAG/ATTGYesN/A
 22FA/GTAGC/TA/TA/GYesN/A
 31FA/GT/CAGC/TA/TA/GYesN/A
 56FA/GTAGC/TA/TA/GYesN/A
 63FGTA/GG/ATTGYesN/A
 68FGTAG/ATTGYesN/A
 78FAT/CAGCAAYesN/A
 82FAT/CAGCAAYesN/A
 59FGTA/GGTTGYesN/A
Normals
 F4GTGGTTGN/AN/A
NheI cloneATAGCAAN/AN/A
  • a ND, not done; N/A, not appropriate.