Table 1

MORF4 sequence alleles

MORF4 sequence at polymorphic loci in tumor-derived cell lines, normal fibroblast counterparts (i.e., 3F fibroblasts were derived from the same patient as the BICR 3 line, and so forth) and “normal” material used for microcell transfer (F4)/transfection (cloned NheI fragment; see Ref. 7 ). Loci base numbering is as used in Fig. 2 <\$REFLINK> . Where two bases are indicated at one position, the sequence was found to be heterozygous at this locus; i.e., two different allelic sequences are present in the same cells. The final column indicates lines that are heterozygous and not homo- nor hemizygous for MORF4 and the 4q33-q34 LOH locus in the vicinity of D4S408.
Base at polymorphic lociHeterozygous
49108115428615730865 MORF4 D4S408
Tumor lines
BICR-3A/GTA/GGC/TA/TA/GYesYes
BICR-6ATAGCAANoNo
BICR-10AT/CAGCAAYesnNDa
BICR-16GTAGTTGNoND
BICR-18GTAGTTGNoNo
BICR-19A/GT/CA/GGCA/TA/GYesNo
BICR-22A/GTAGC/TA/TA/GYesNo
BICR-31ACAGCAANoNo
BICR-56A/GTAGC/TA/TA/GYesND
BICR-63GTA/GG/ATTGYesYes
BICR-68GTAG/ATTGYesYes
BICR-78ACAGCAANoNo
BICR-82ATAGCAANoNo
SCC-4AT/CAGCAAYesND
SCC-9GTA/GGTTGYesND
SCC-12A/GT/CA/GGC/TA/TA/GYesND
SCC-13A/GT/CAGCAAYesND
SCC-15A/GTA/GGCA/TA/GYesND
SCC-25ATAGCAANoND
SV-HFKATAGCAANoND
DOKACAGCAANoND
Fibroblasts
3FA/GTA/GGC/TA/TA/GYesN/A
6FAT/CAGCAAYesN/A
18FGTAG/ATTGYesN/A
22FA/GTAGC/TA/TA/GYesN/A
31FA/GT/CAGC/TA/TA/GYesN/A
56FA/GTAGC/TA/TA/GYesN/A
63FGTA/GG/ATTGYesN/A
68FGTAG/ATTGYesN/A
78FAT/CAGCAAYesN/A
82FAT/CAGCAAYesN/A
59FGTA/GGTTGYesN/A
Normals
F4GTGGTTGN/AN/A
NheI cloneATAGCAAN/AN/A
• a ND, not done; N/A, not appropriate.