Table 1

PLAG1 can stimulate transcription through its consensus binding site

Two hundred ng of the indicated reporter luciferase (luc) construct were cotransfected into the fetal kidney cell line 293 together with 200 ng of the expression vector pCAGGS-PLAG1 or the empty vector pCAGGS. PLAG1 induction levels are expressed as the ratio of luciferase activity obtained in the cell transfected with pCAGGS-PLAG1 expression vector versus the activity obtained in cells transfected with the empty vector pCAGGS. The data are means ± SE of at least two independent transfection experiments, each performed in triplicate.

PLAG1 induction
(WT)6-TK-luc18.9 ± 2.6
(mCLU)6-TK-luc1.0 ± 0.2
(mCO)6-TK-luc0.9 ± 0.2
(mCLUmCO)6-TK-luc0.6 ± 0.1
TK-luc1.7 ± 0.6