Table 2

Identification of human promoters with potential PLAG1 binding sites and determination of their capacity to be induced by PLAG1

The EPD (18) has been screened for the presence of the PLAG1 consensus GRGGC(N)7 RGGK with the pattern-matching algorithm implemented in the program findpatterns of the GCG software package (32). The list includes all of the human promoters present in the EPD containing at least two PLAG1 DNA binding consensus in their promoter. The ability of each promoter to be induced by PLAG1 has been estimated by cotransfection of the fetal kidney 293 cell line with pCAGGS-PLAG1 or pCAGGS expression vectors, together with reporter constructs in which each promoter has been cloned in front of a luciferase gene (see“ Materials and Methods”). PLAG1 induction levels are expressed as the ratio of luciferase activity obtained in the cell transfected with pCAGGS-PLAG1 expression vector versus the activity obtained in cells transfected with the empty vector pCAGGS. The data are means ± SE of at least two independent transfection experiments, each performed in triplicate.

PLAG1 induction
5 consensus :IGF-II promoter 37.9 ± 0.8
4 consensus :GOS24/TTP/Zfp363.0 ± 0.2
3 consensus :c-Ha-Ras promoter 30.9 ± 0.2
2 consensus :PDGF-B/c-sis1.4 ± 0.6
TnI slowND
GastrinND
VLDLND
SOD-1ND
Ia-ass. g′ND
H19ND
0 consensus :pGL2 basic0.7 ± 0.2
pSLA31.2 ± 0.1
RSV1.1 ± 0.2
prohormone convertase 20.5 ± 0.03
somatostatin1.0 ± 0.3