Table 4

Immunohistochemical analysis of human pancreatic carcinoma in the pancreas of control and treated nude mice

Treatment groupaTumor cellsEndothelial cells
PCNATUNELVEGFIL-8CD31% TUNEL
Saline control237 ± 39b66 ± 21b338 ± 37b296 ± 46c60 ± 230d
PKI166185 ± 33287 ± 84e173 ± 20e185 ± 11e24 ± 18e20e
Gemcitabine237 ± 66225 ± 95e298 ± 54299 ± 4352 ± 180
PKI166+ gemcitabine130 ± 45e298 ± 80e156 ± 31e187 ± 35e24 ± 13e31e
  • a L3.6pl human pancreatic cancer cells (1 × 106) were injected into the pancreas of nude mice. Seven days later, groups of mice were treated with biweekly i.p. injections of gemcitabine (125 mg/kg) alone, daily oral feedings of PKI166 (100 mg/kg) alone, gemcitabine and PKI166, or saline (control). All mice were killed on day 35.

  • b Mean ± SD positive cells/field determined from measurement of 10 random 0.159-mm2 fields at× 100.

  • c Mean ± SD absorbance determined as described in “Materials and Methods.”

  • d Mean ± SD CD31/TUNEL-positive cells in 10 random 0.011-mm2 fields at ×400. Fluorescent double labeling was performed on frozen tissue sections.

  • e P < 0.001 as compared with controls.