Table 2

Effects of Se-methylselenocysteine and methylseleninic acid on DNA damage as measured by comet assay in TM2H and TM12 cellsa

TreatmentTM2H cellsTM12 cells
None78 ± 6.059 ± 2.0
Se-methylselenocysteine
25 μm49 ± 4.056 ± 3.0
50 μm51 ± 6.056 ± 3.0
None79 ± 6.059 ± 1.7
Methylseleninic acid
2.5 μm55 ± 3.558 ± 1.3
5 μm54 ± 4.162 ± 3.9
  • The data are expressed as arbitrary units of alkaline-labile DNA damage, mean ± SE (n = 9). The score ranges from a minimum of zero to a maximum of 400. All of the results were obtained from 24-h cultures. The data were evaluated statistically to test the hypothesis that treatment with either Se-methylselenocysteine or methylseleninic acid would increase alkaline-labile DNA damage. No evidence was found to support this hypothesis.