Table 2

Expression of the skin-homing receptor CLA on CD3+ T cells in peripheral blood of patients receiving rHuIL-12

Expression of CLA antigen (on green fluorescence histograms) was evaluated by two-color immunofluorescence after gating on T cells stained by PE-conjugated anti-CD3 mAbs on red fluorescences versus forward-scatter plots. PBL samples were taken before (day −4) rHuIL-12 administration; 1, 4 and 7 days after the first cytokine injection; and 1 and 4 days after the last injection (days +43 and +47).

DayPatient no.
789
−48.5a13.015.6
+116.3b23.0b29.0b
+47.319.5b24.2b
+711.416.823.0b
+431.8b19.8b19.1
+479.419.6b17.5
  • a Results expressed as percentage of CLA+ cells within the CD3+ fraction.

  • b Difference in proportion of CLA+ T cells in comparison with day −4 FL-2 histograms is significant (P < 0.01; Kolmogorov-Smirnov statistics for each comparison).