Table 3

Effect of NS398 on cell cycle distributiona

Solvent blankAverage (n = 6)57.5623.4019.04
NS398Average (n = 6)55.3817.5827.05
  • VAMT-1 cells were exposed to 87 μm NS398 or a solvent control for 6 days and harvested for analysis of apoptotic fraction and cell cycle distribution parameters as described in “Materials and Methods.” Collected cells were fixed in 70% EtOH and treated with propidium iodide. Analyses were performed on a Becton Dickinson FACSCalibur instrument with data collection by the Cell Quest program. Analysis of cell cycle distribution parameters was performed using the ModFit program.