Table 3

Effect of NS398 on cell cycle distributiona

G0-G1SG2-M
Solvent blankAverage (n = 6)57.5623.4019.04
SD1.252.082.34
NS398Average (n = 6)55.3817.5827.05
SD1.090.900.95
  • VAMT-1 cells were exposed to 87 μm NS398 or a solvent control for 6 days and harvested for analysis of apoptotic fraction and cell cycle distribution parameters as described in “Materials and Methods.” Collected cells were fixed in 70% EtOH and treated with propidium iodide. Analyses were performed on a Becton Dickinson FACSCalibur instrument with data collection by the Cell Quest program. Analysis of cell cycle distribution parameters was performed using the ModFit program.