Table 1

Edman degradation profile of the 78-kDa protein cross-linked by photoactivated GV

The yield of the respective amino acid residue in each cycle is presented in pmol. The amino acid that exhibited the largest recovery is underlined. Amino acid residues are abbreviated as follows: D, aspartic acid; E, glutamic acid; N, asparagine; S, serine; T, threonine; Q, glutamine; G, glycine; H, histidine; A, alanine; Y, tyrosine; R, arginine; P, proline; M, methionine; V, valine; W, tryptophane; K, lysine; F, phenylalanine; I, isoleucine; L, leucine.

Amino acidCycle no.
1234567891011121314151617
D2.63.4 36.1 2.72.93.8 33.2 3.12.42.02.62.93.63.1 22.4 2.42.0
E 33.4 30.6 4.93.63.0 32.7 3.93.02.42.02.53.63.02.12.43.42.7
N2.12.62.00.91.81.62.82.41.41.21.82.41.32.52.11.41.6
S1.11.62.20.61.32.11.61.82.42.31.31.82.03.12.11.61.8
T0.81.11.60.41.71.40.71.61.3 19.3 2.52.01.51.82.01.21.8
Q3.52.61.82.02.62.41.81.41.61.92.02.72.41.81.41.91.8
G4.24.14.03.83.93.63.43.2 28.8 5.64.03.6 23.5 3.94.13.5 23.4
H1.60.80.71.61.11.20.90.81.82.02.41.51.71.90.71.21.6
A4.13.53.93.24.43.73.23.32.93.74.44.23.63.12.93.83.9
Y1.51.31.00.81.11.51.62.01.61.92.92.52.01.52.71.81.6
R3.22.82.93.01.92.72.93.03.43.72.72.23.63.21.92.53.7
P2.93.02.22.12.62.33.03.43.62.92.22.62.52.11.92.62.9
M2.52.11.82.01.30.60.91.42.12.42.61.61.82.12.52.21.5
V3.83.12.12.73.12.03.6 25.8 5.04.1 22.4 20.9 4.94.13.43.12.7
W00000000000000000
K2.42.10.9 19.8 17.8 3.93.12.01.92.12.32.61.41.42.82.32.1
F1.91.20.70.41.31.21.01.52.42.11.51.51.22.31.21.61.8
I3.53.12.12.82.52.11.92.12.93.12.72.41.4 23.8 4.63.12.8
L2.52.11.51.71.81.10.80.41.32.52.62.32.22.61.7 21.6 3.4