Morphological changes in KG-1 and THP-1 cells after treatment with ATRA and/or GM-CSF for 9 days
Morphological maturation was classified according to the nuclear shape as blast, myelocyte, banded, and segmented. Differential counts were performed under a microscope among a minimum of 200 cells. Morphological changes were determined as described in “Materials and Methods.” Values are the mean ± SD of triplicate determinations.
| Inducer | Myeloid cell type (% of total cells ± SD) | |||
|---|---|---|---|---|
| Blasts | Myelocytes | Banded neutrophils | Segmented neutrophils | |
| KG-1 | ||||
| None | 85.8 ± 2.0 | 14.2 ± 2.0 | 0.0 | 0.0 |
| GM-CSF (0.1 ng/ml) | 88.0 ± 3.6 | 12.0 ± 3.6 | 0.0 | 0.0 |
| ATRA (10−7 m) | 24.7 ± 2.4 | 50.0 ± 2.9 | 16.0± 2.7 | 9.3± 0.6 |
| GM-CSF+ ATRA | 7.0 ± 1.8 | 21.0 ± 1.8 | 29.7± 2.1 | 42.3± 2.4 |
| THP-1 | ||||
| None | 98.7 ± 0.2 | 1.3 ± 0.2 | 0.0 | 0.0 |
| GM-CSF (0.1 ng/ml) | 90.7 ± 0.8 | 9.3 ± 0.8 | 0.0 | 0.0 |
| ATRA (10−7 m) | 12.7 ± 2.2 | 31.1 ± 1.3 | 38.5± 2.9 | 17.7± 0.6 |
| GM-CSF+ ATRA | 4.7 ± 2.1 | 17.2 ± 3.0 | 28.3± 4.3 | 49.8± 3.2 |