Table 1

Morphological changes in KG-1 and THP-1 cells after treatment with ATRA and/or GM-CSF for 9 days

Morphological maturation was classified according to the nuclear shape as blast, myelocyte, banded, and segmented. Differential counts were performed under a microscope among a minimum of 200 cells. Morphological changes were determined as described in “Materials and Methods.” Values are the mean ± SD of triplicate determinations.

InducerMyeloid cell type (% of total cells ± SD)
BlastsMyelocytesBanded neutrophilsSegmented neutrophils
KG-1
None85.8 ± 2.014.2 ± 2.00.00.0
GM-CSF (0.1 ng/ml)88.0 ± 3.612.0 ± 3.60.00.0
ATRA (10−7 m)24.7 ± 2.450.0 ± 2.916.0± 2.79.3± 0.6
GM-CSF+ ATRA7.0 ± 1.821.0 ± 1.829.7± 2.142.3± 2.4
THP-1
None98.7 ± 0.21.3 ± 0.20.00.0
GM-CSF (0.1 ng/ml)90.7 ± 0.89.3 ± 0.80.00.0
ATRA (10−7 m)12.7 ± 2.231.1 ± 1.338.5± 2.917.7± 0.6
GM-CSF+ ATRA4.7 ± 2.117.2 ± 3.028.3± 4.349.8± 3.2