Table 1

Invasion of HSPG-coated filters in brain-metastatic melanoma cells: effects of cortical astrocytes CM

ConditionsTime (h)Brain-metastatic melanoma cells
70WB16B15b
Cells alone01.00 ± 0.1a1.13 ± 0.2
722.84 ± 0.93.02 ± 0.7
+ ACM01.10 ± 0.51.08 ± 0.3
726.81 ± 0.77.83 ± 0.3
+ CM, NGF-treated astrocytes01.02 ± 0.21.10 ± 0.5
7220.7 ± 0.624.3 ± 0.2
+ CM, HEP-treated astrocytes01.08 ± 0.31.07 ± 0.2
721.02 ± 0.21.02 ± 0.1
n = 4bn = 4
  • a The values given are arbitrary fluorescence units. The fluorescence units at time 0 were set to 1.00. Chemoinvasion assays were performed as reported (2).

  • b Data are the means of four independent experiments with quadruplicate assays performed in each experiment; P < 0.01.