Table 1

Effect of DFMO on the morphology of prostate and GU weight in TRAMP mice and their nontransgenic littermatesa

GroupbNo. of AnimalsProstate cancerAnimals with metastasiscProstate weight (mg)Prostate volume (mm3)GU weight (g)
LymphLungs
Experiment 1
Nontransgenic water-fed8None0 /8 (0%)0 /8 (0%)12.9 ± 1.18.47 ± 0.530.38 ± 0.05
Nontransgenic DFMO-fed8None0 /8 (0%)0 /8 (0%)11.4 ± 1.47.62 ± 0.490.34 ± 0.03
Transgenic water-fed8Severe7 /8 (87.5%)6 /8 (75%)63.2 ± 8.446.92 ± 9.122.69 ± 0.48
Transgenic DFMO-fed8Mild0 /8 (0%)0 /8 (0%)24.6 ± 2.4d16.36 ± 1.54d0.94 ± 0.11d
Experiment 2
Nontransgenic water-fed8None0 /8 (0%)0 /8 (0%)11.6 ± 1.07.87 ± 0.480.32 ± 0.04
Nontransgenic DFMO-fed8None0 /8 (0%)0 /8 (0%)10.8 ± 1.27.16 ± 0.440.29 ± 0.04
Transgenic water-fed8Severe8 /8 (100%)5 /8 (62.5%)76.4 ± 10.254.64 ± 9.683.12 ± 0.52
Transgenic DFMO-fed8Mild0 /8 (0%)0 /8 (0%)30.5 ± 3.6d18.22 ± 2.08d1.16 ± 0.12d
Cumulative
Nontransgenic water-fed16None0 /8 (0%)0 /8 (0%)12.2 ± 1.28.14 ± 0.520.35 ± 0.05
Nontransgenic DFMO-fed16None0 /8 (0%)0 /8 (0%)11.2 ± 1.57.38 ± 0.500.31 ± 0.04
Transgenic water-fed16Severe15 /16 (93.7%)11 /16 (68.7%)67.8 ± 9.850.76 ± 9.462.90 ± 0.50
Transgenic DFMO-fed16Mild0 /16 (0%)0 /16 (0%)27.5 ± 3.2d17.28 ± 1.88d1.06 ± 0.12d
  • a The data represented in two independent experiments is the mean ± SE of eight mice.

  • b Mice (8 weeks of age) were given plain drinking water (for the control group) or DFMO in drinking water for 20 weeks. At the age of 28 weeks, the animals were sacrificed and studied for prostate tumorigenesis and metastasis.

  • c Metastasis in the lymph was examined microscopically, whereas metastasis in lungs was examined by india ink method as described in “Materials and Methods.”

  • d P < 0.001, water-fed control TRAMP compared with DFMO-treated TRAMP mice, Student’s t test.