Table 1

Tumorigenicity, vascularization, production of ascites, and survival of nude mice given peritoneal injections of IκB αM-transfected human ovarian cancer cells

One million viable cells were injected into the peritoneal cavity of female nude mice (n = 10). The mice were killed when they became moribund or 90 days after tumor cell injection. The volume of malignant ascites was measured. Solid tumors in the peritoneal cavity were resected and weighed. Mean vessel density was determined by immunohistochemistry of tumor sections using CD31 antibody.

CellsTumorigenicityVessel densityb (Mean ± SD)Survival Median days (range)
Solid tumorsAscites Mean volume ± SD (ml)
IncidenceaMean weight ± SD (g)
SKOV3ip.110 /103.2 ± 0.52.3 ± 0.778 ± 1545 (32–53)
SKOV3ip.1-Neo10 /102.9 ± 0.42.6 ± 0.571 ± 1647 (38–60)
SKOV3ip.1-IM14 /100.6 ± 0.8c0.3 ± 0.7b27 ± 6c90 (69−>90)b
SKOV3ip.1-IM28 /101.3 ± 0.7c0.6 ± 0.6b37 ± 8c73 (54−>90)b
HEY-A810 /102.7 ± 0.7NAd111 ± 2334 (26–42)
HEY-A8-Neo10 /103.3 ± 0.5NA132 ± 3230 (21–51)
HEY-A8-IM19 /101.6 ± 0.8cNA58 ± 14c68 (55−>90)c
HEY-A8-IM27 /101.3 ± 1.1cNA49 ± 10c77 (67−>90)c
  • a Mice with i.p. tumor/mice that received injections.

  • b Mean number of vessels ±SD per 10× 200 fields.

  • c P < 0.001, Mann-Whitney U test.

  • d No ascites.