Table 2

Effect of estrone derivatives on mitosis in MCF7 cells

MCF7 cells were treated with the indicated compounds for 24 h. Cells were recovered by trypsinization, and cytospins were prepared. DNA was stained with PI, and cells in mitosis (i.e., with condensed chromosomes) were determined as a percentage of total cells.

CompoundMitotic cells
Control4%
2-EtEMATE, 5 μm49%
2-EtEMATE, 500 nm29%
2-MeOEMATE, 5 μm38%
2-MeOEMATE, 500 nm26%
2-EtE1, 5 μm6%
2-EtE1, 500 nm4%