Table 3

Effects of 1 and 2 h of 1 μg/ml actinomycin D (ActD) on the stability of RAR-α and RAR-γ mRNA in untreated or retinoid-treated (48 h) SK-BR-3 cells

RetinoidConcentration (m)RAR-α mRNA (% of control)RAR-γ mRNA (% of control)
Pre-ActDPost-ActDSFa (post/pre)Pre-ActDPost-ActDSFa (post/pre)
1 h2 h1 h2 h1 h2 h1 h2 h
9cRA10166,8109156,50.650,94157,5112152,50,710,97
tRA10181,71591980.881,09185,71501340,810,72
TTNPB10210,31731820.820,871551451680,941,08
AM8010203,31342020.660,991691191920,701,14
AM58010220,71341390.610,631851331380,720,75
CD437101501441090.960,73138,31351060,980,77
Ro 25-738610135,51221570.91,16117,71241171,050,99
  • a SF, stability factor; SF >1, treatment elevates RNA stability; SF <1, treatment reduces RNA stability; SF = 1, treatment does not affect RNA stability.