Table 3

Role of Myc in ODC promoter-driven luciferase activity

A fragment (−462 to +3070) of the human ODC gene was isolated by PCR from individuals homozygous for either the A or G allele and cloned into the pGL3 plasmid (Promega) in front of a modified firefly luciferase gene. YY8ME4 cells (21) were transiently transfected using Lipofectin reagent with the above constructs (1 μg) along with 0.1 μg of a renilla luciferase plasmid (pRL; Promega). In some dishes, 0.1 μm 4-HT was added 3 h prior to harvesting cells for luciferase assay. Luciferase activities were measured in cell extracts 36 h post-transfection. Values in parentheses indicate the percentage of increase in luciferase activity produced by 4-HT treatment.

ExperimentLuciferase activitya
A alleleG allele
−4HT+4HTΔ4-HT (%)−4HT+4HTΔ4HT (%)
121.330.39.0 (42)5.76.40.7 (12)
238.759.821.1 (55)4.45.00.6 (14)
  • a Ratio of firefly relative luminescence units/μg of protein to renilla luminescence units/μg of protein measured in the same extract.