Table 1

The primer sequences used for PCR-SSCP analysis

GenesExonForwardReverseRestriction enzyme
APC a  15′-CACGCAGCTACTCCATTTTT-3′5′-TTACAAGAGGAAACACTGAA-3′
 25′-GGTTATTTAGCATTTCATGC-3′5′-TCTCTAAAGTCCTTTAAAAA-3′
 35′-GGCATAAGCAGTTACTACAT-3′5′-GAGCTCCCGTACACAAGAGT-3′
 45′-GCTCTCACAAGTCCATTGCT-3′5′-AAGTCCCCAAATCTAAAAGC-3′
 55′-AAATTAAGTGCTCTTTGTGG-3′5′-GGCAGAGCTGTCAGTGTGCA-3′
 65′-ATCTGACTTGATTCTCTTTT-3′5′-CACACGCATGTAACAAAAGG-3′
 75′-CGGTTTTGTACATAACGATA-3′5′-TTTCCAGGGGCTCACATTCT-3′
 85′-CTTCTAGACTGAGAGTGTCA-3′5′-CATAAAGACCGAGTGAGAAC-3′
 95′-GATCTGGCTTTTGGGTATTA-3′5′-TTGAAACATGCATTGTGATG-3′PvuII, Hinfl
105′-AGCATTATGGCTTAGGTGAC-3′5′-ATTATCTGTGGCATTACAAA-3′
115′-CAGTTTATTCGATTATGGAT-3′5′-AGCACCTTAGGAAGTAAATC-3′
125′-GCCCCTTGATACTACTCT-3′5′-AAAAGCCACATCAGAAAT-3′
135′-AAAAAGGCAGTTAGTATGAA-3′5′-TTAATACCAGCTCAGAGAGG-3′
145′-TTTATAGAAACAGCACTGAA-3′5′-CGTTCTAAAGAAACAAACTC-3′MspI
15A5′-TGTCACTAACCACATTTCCT-3′5′-AGCTGCTGCGCTCCCCATAG-3′
15B5′-CAGGAGGCTCTGTGGGACAT-3′5′-TGCTTACTGCGATGAGATGC-3′
15BD5′-TTTATCAGAAACCTTTGACA-3′5′-TTACCATAGCCATCACTACT-3′HaeIII, MvaI
15DE5′-GACATGCTCCATGCCTTATG-3′5′-CATGGTGTTTCTCTTCATTA-3′RsaI, TagI
15EG5′-GAAGATGACAAGCCTACCAA-3′5′-AGAGTCTGCCTCCTGTGTTG-3′RsaI
15FH5′-TTTCTCAAGGTGTAGTTCCT-3′5′-TTGCTTCTGCTTGGTGGCAT-3′MspI
15HI5′-TTCTGTCAGCTCCCTGGACA-3′5′-TCGGAATCATCTAATAAGTC-3′MspI
β-Catenin b 5′-GCTGACCTGATGGAGTTGGA-3′5′-GCTACTTGCTCTTGCGTGAA-3′
  • a–b These sequences were previously reported in:

  • a Refs. 28 and 29; and

  • b Ref. 23 .