The primer sequences used for PCR-SSCP analysis
| Genes | Exon | Forward | Reverse | Restriction enzyme |
|---|---|---|---|---|
| APC a | 1 | 5′-CACGCAGCTACTCCATTTTT-3′ | 5′-TTACAAGAGGAAACACTGAA-3′ | |
| 2 | 5′-GGTTATTTAGCATTTCATGC-3′ | 5′-TCTCTAAAGTCCTTTAAAAA-3′ | ||
| 3 | 5′-GGCATAAGCAGTTACTACAT-3′ | 5′-GAGCTCCCGTACACAAGAGT-3′ | ||
| 4 | 5′-GCTCTCACAAGTCCATTGCT-3′ | 5′-AAGTCCCCAAATCTAAAAGC-3′ | ||
| 5 | 5′-AAATTAAGTGCTCTTTGTGG-3′ | 5′-GGCAGAGCTGTCAGTGTGCA-3′ | ||
| 6 | 5′-ATCTGACTTGATTCTCTTTT-3′ | 5′-CACACGCATGTAACAAAAGG-3′ | ||
| 7 | 5′-CGGTTTTGTACATAACGATA-3′ | 5′-TTTCCAGGGGCTCACATTCT-3′ | ||
| 8 | 5′-CTTCTAGACTGAGAGTGTCA-3′ | 5′-CATAAAGACCGAGTGAGAAC-3′ | ||
| 9 | 5′-GATCTGGCTTTTGGGTATTA-3′ | 5′-TTGAAACATGCATTGTGATG-3′ | PvuII, Hinfl | |
| 10 | 5′-AGCATTATGGCTTAGGTGAC-3′ | 5′-ATTATCTGTGGCATTACAAA-3′ | ||
| 11 | 5′-CAGTTTATTCGATTATGGAT-3′ | 5′-AGCACCTTAGGAAGTAAATC-3′ | ||
| 12 | 5′-GCCCCTTGATACTACTCT-3′ | 5′-AAAAGCCACATCAGAAAT-3′ | ||
| 13 | 5′-AAAAAGGCAGTTAGTATGAA-3′ | 5′-TTAATACCAGCTCAGAGAGG-3′ | ||
| 14 | 5′-TTTATAGAAACAGCACTGAA-3′ | 5′-CGTTCTAAAGAAACAAACTC-3′ | MspI | |
| 15A | 5′-TGTCACTAACCACATTTCCT-3′ | 5′-AGCTGCTGCGCTCCCCATAG-3′ | ||
| 15B | 5′-CAGGAGGCTCTGTGGGACAT-3′ | 5′-TGCTTACTGCGATGAGATGC-3′ | ||
| 15BD | 5′-TTTATCAGAAACCTTTGACA-3′ | 5′-TTACCATAGCCATCACTACT-3′ | HaeIII, MvaI | |
| 15DE | 5′-GACATGCTCCATGCCTTATG-3′ | 5′-CATGGTGTTTCTCTTCATTA-3′ | RsaI, TagI | |
| 15EG | 5′-GAAGATGACAAGCCTACCAA-3′ | 5′-AGAGTCTGCCTCCTGTGTTG-3′ | RsaI | |
| 15FH | 5′-TTTCTCAAGGTGTAGTTCCT-3′ | 5′-TTGCTTCTGCTTGGTGGCAT-3′ | MspI | |
| 15HI | 5′-TTCTGTCAGCTCCCTGGACA-3′ | 5′-TCGGAATCATCTAATAAGTC-3′ | MspI | |
| β-Catenin b | 5′-GCTGACCTGATGGAGTTGGA-3′ | 5′-GCTACTTGCTCTTGCGTGAA-3′ |
a–b These sequences were previously reported in:
a Refs. 28 and 29; and
b Ref. 23 .