Table 1

Summary of the mutations identified in group 2B

TumorExonMutationAmino acid changeNo. of PCR-SSCP amplificationsNo. of mutated templatesaNo. of templates sequenced
15GCC-ACCA-748-T3812
17TCC-CCCS-865-P3811
17CAG-TAGQ-867-stop3416
18CAG-CGGQ-919-R3320
32GCC-GTCA-586-V3320
52AAG-AGGK-580-R3320
87AGA-GGAR-846-G3813
112ACA-GCAT-575-A3515
122GCT-TCTA-587-S3320
183TGT-TATC-619-Y3618
205GTC-GCCV-757-A3517
  • a In every case, wild-type sequences were also identified in other templates, indicating that each mutation was a somatic event. Each of these mutations was identified in at least three separate PCR amplifications.